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Annotating Cluster C1 phage onn Macbook Pro

Link to this post \| posted 04 Jun, 2018 18:16
powelleann	Hello, Is there any way to get a large cluster C1 phage genome to annotate on a MacBook Pro (using WINE). Most of the time, I can open the file but when I try to show ORFs in a frames view I run out of memory or get various error messages. Another time I was able to generate the ORFs in Frames, but then could not get RBS scores when I selected a gene (again an error message). I will be using this computer during the upcoming faculty workshop, so any help would be much appreciated. Ann

Link to this post | posted 04 Jun, 2018 18:16

powelleann

Hello,

Is there any way to get a large cluster C1 phage genome to annotate on a MacBook Pro (using WINE). Most of the time, I can open the file but when I try to show ORFs in a frames view I run out of memory or get various error messages. Another time I was able to generate the ORFs in Frames, but then could not get RBS scores when I selected a gene (again an error message).

I will be using this computer during the upcoming faculty workshop, so any help would be much appreciated.

Ann

Link to this post \| posted 04 Jun, 2018 21:12
DanRussell	Hi Ann, I'm not sure there is. My recent experiences with WINE have varied, and almost always the size of the genome is a determining factor in how well WINE works. A 50 kb genome loads in a few seconds, but a 100 kb genome never works. Not helpful, obviously, but others may have better suggestions. You may be able to request a loaner laptop for the workshop. I'd ask Vic and/or Billy. –Dan