SEA-PHAGES | methyltransferase/glycosyltransferase

Link to this post \| posted 02 Aug, 2017 19:26
dbolliva	Pham 6811 (with only three members, all J) appears to have two enzyme functions in the same polypeptide. The amino terminal region has strong HHpred results for methyltransferases (probability 98%) and also hits in CDD. The C terminus has hits on galactosyltransferases and glycosyltransferases (probability 99.7% for both) and also has hits in the CDD though the CDD hit is to glycosyltransferases. Currrently there is no ability to call combined methyltransferase/glycosyltransferase but it seems very likely that this is the case here. Can this be added? I have linked a copy of the HHpred result 58Kb

Link to this post | posted 02 Aug, 2017 19:26

Pham 6811 (with only three members, all J) appears to have two enzyme functions in the same polypeptide. The amino terminal region has strong HHpred results for methyltransferases (probability 98%) and also hits in CDD. The C terminus has hits on galactosyltransferases and glycosyltransferases (probability 99.7% for both) and also has hits in the CDD though the CDD hit is to glycosyltransferases. Currrently there is no ability to call combined methyltransferase/glycosyltransferase but it seems very likely that this is the case here. Can this be added? I have linked a copy of the HHpred result

Link to this post \| posted 03 Aug, 2017 15:18
welkin	Sure. I wonder if there is a way to shorten the name and remain clear.

Link to this post \| posted 07 Jul, 2018 17:09
plymaler	ThetaBob is an F4 phage. ThetaBob_107 has good HHPred matches to both glycosyltransferases and methyltransferases; screenshots of the HHPred output are attached. A combined glycosyltransferase/methytransferase function is not on the approved function list and so I am not sure how to identify the function of this protein. It does not seem to lean one way or the other. ThetaBob_107 is currently in pham 3147 with 9 other non-draft proteins, all in F phage. Avani_105, Jabbawokkie_106, Zapner_106, WillSterrel_99, Llama_108, and Demsculpinboyz_115 are all labeled as glycosyltransferase/methyltransferase. Che9d_109 and Yoshi_114 are labeled as glycosyl transferase. Che8_108 is unlabeled. Can glycosyltransferase/methytransferase be added an approved function? If not, what is the recommendation for how to label ThetaBob_107? Thanks! 352Kb

Link to this post | posted 07 Jul, 2018 17:09

plymaler

ThetaBob is an F4 phage. ThetaBob_107 has good HHPred matches to both glycosyltransferases and methyltransferases; screenshots of the HHPred output are attached. A combined glycosyltransferase/methytransferase function is not on the approved function list and so I am not sure how to identify the function of this protein. It does not seem to lean one way or the other.

ThetaBob_107 is currently in pham 3147 with 9 other non-draft proteins, all in F phage. Avani_105, Jabbawokkie_106, Zapner_106, WillSterrel_99, Llama_108, and Demsculpinboyz_115 are all labeled as glycosyltransferase/methyltransferase. Che9d_109 and Yoshi_114 are labeled as glycosyl transferase. Che8_108 is unlabeled.

Can glycosyltransferase/methytransferase be added an approved function? If not, what is the recommendation for how to label ThetaBob_107?

Thanks!

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methyltransferase/glycosyltransferase