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Ruth Plymale posted in Tenure track Microbiology faculty position at Ouachita Baptist University in Arkansas
bburnes posted in Calling start in FC phage Phrampa
cdshaffer posted in Calling start in FC phage Phrampa
bburnes posted in Calling start in FC phage Phrampa
Debbie Jacobs-Sera posted in Calling start in FC phage Phrampa
% alignment
| Link to this post | posted 11 Feb, 2021 20:00 | |
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For the BLASTp analysis done in assessing the start site, should we be reporting % SUBJECT or QUERY aligned? DNA Master reports % SUBJECT aligned but NCBI lists % QUERY coverage. For most cases, this will not matter as they will be at or near 100% aligned for both query and subject. Or maybe it does not matter for this purpose, when it is used to help evaluate start site choice? I don't recall being asked this in the past or having a discussion about it. We have always used the % SUBJECT aligned value from DNA Master except when we went to NCBI or phagesdb. I cannot find a clear statement about which should be recorded. |
| Link to this post | posted 15 Feb, 2021 17:07 | |
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Joe, It doesn't matter, just state (maybe in your cover letter) what you used. debbie |