Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Recent Activity

EA1 tRNA

Link to this post \| posted 25 Feb, 2019 03:35
smg366	We are annotating some EA1 phages (host was Microbacterium foliorum). In phage PrincePhergus both Aragorn and tRNAscan predict 1 tRNA. We want to call this tRNA, but when I compare it to phages Golden and Koji on PECAAN, they both show 1 tRNA predicted by both Aragorn and tRNAscan, but they don't call it in their final annotation. My impression was that we call any tRNA gene which was identified by Aragorn. Is there some reason that it was not called in these other phages? Thanks, Susan

Link to this post | posted 25 Feb, 2019 03:35

smg366

We are annotating some EA1 phages (host was Microbacterium foliorum). In phage PrincePhergus both Aragorn and tRNAscan predict 1 tRNA. We want to call this tRNA, but when I compare it to phages Golden and Koji on PECAAN, they both show 1 tRNA predicted by both Aragorn and tRNAscan, but they don't call it in their final annotation.

My impression was that we call any tRNA gene which was identified by Aragorn. Is there some reason that it was not called in these other phages?

Thanks,
Susan

Link to this post \| posted 25 Feb, 2019 22:26
debbie	Susan, Excellent question! I think those tRNAs are missed in Golden and Koji. I will be making some edits soon. Good catch! debbie

Link to this post \| posted 25 Feb, 2019 23:27
smg366	Thank you, I will make sure they are included in our phages. Susan