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Submit to GenBank Error
| Link to this post | posted 10 Sep, 2018 15:23 | |
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DNAMaster recently updated on my PC and I am no longer able to open the 'Submit to GenBank' window. The version is 5.23.2, Build 2592, 26 June 2018. I am running with Admin privileges and can open a dnam5 and all looks and works fine. When I click on Tools - Submit to GenBank, I get the error message "BLOB has has been modified." Didn't have this problem last week. Any suggestions to 'unmodify' the BLOB? Best, Denise |
| Link to this post | posted 10 Sep, 2018 18:22 | |
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Sally had this error and solved it by deleting all projects; see the last couple of posts in this thread: https://seaphages.org/forums/topic/17/?page=8 |
| Link to this post | posted 10 Sep, 2018 19:07 | |
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Denise, I knew I knew something about this but it took me a few prompts (thanks, Chris) to recall anything. It might be helpful to go to DMDB folder of the DNA Master application folder. there should be a GBS folder for each project. if you have no projects, you should not have any folders, so I am not sure that will fix anything. In July, I had the same problem. Here is what Jeffrey had me do to fix it. However, I couldn't fix and and sent him files. He eventually got it fixed, so I am not sure this will help. According to Jeffrey, the BLOB error means: That means the database tables are not synchronized; each table has multiple files, and premature closing of a program or other database failure can cause them to get out of sync. Close DNA Master Go to the program directory and open the Helper directory Run the dtutil32 program. This is an immensely useful program. Repeat steps below for any database errors. Click the “By Directory” button In the dialog, go to the DMDB directory and open the GenbankProjects.db file Click the Verify button Click the Rebuild button Note any reports that the verification or rebuild was not successful. If so, repeat steps 7 then 6 on the file. If there is corruption, they should rebuild without error, so no errors reported does not mean nothing is working. Repeat steps 4-8 for the GenBankAuthors and GenBankReferences files Restart DNA Master Those are the DB files for GenBank projects and are the most likely files with the out-of-sync entries. Me gain - So I tried this, like I said and couldn't make it work. since then, though, I have corrupted a file or 2. I sought out the GBsubmit folder and deleted the specific folder for a genome. When I did this, I corrupted an 'old' project, so i think it is still applicable to your problem… I know this isn't very helpful, but maybe in wiser hands than mine it will work… You are welcome to call. debbie |
| Link to this post | posted 11 Sep, 2018 22:59 | |
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Hi Deb and Chris - Thank you for the rapid replies. I tried the recommended fix and no luck. The 'Verify' button indicated all was ok but, unfortunately, the "Rebuild" button did not successfully restore the files or remove the error message. I tried to verify and rebuild all 3 of the files recommended in the post and in different orders to no avail. I also tried deleting all of the GenBank packages and it still would not even allow me access anything when I clicked "Submit to GenBank' from the Tools menu. Alas (with one small tear), I uninstalled DNA Master and re-installed again. I lost my GenBank packages but I had all of the files so I could re-create them again. Hope the BLOB doesn't strike anyone else! Best, Denise |
| Link to this post | posted 24 Jun, 2020 21:17 | |
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I just ran into the same problem and forgot to check the forum but contacted Jeffrey Lawrence directly. I asked him what the BLOB error stood for and how to fix it. Here is his reply: >>>>> It means the database file was corrupted. Most times, this can be fixed. This is esoteric, but give it a try: Close DNA Master Go to the Helper directory in the DNA Master installation directory Look for a program called “dutil32.exe”. Run it. In the top box, click the “By Directory” button Navigate to the DNA master installation directory, then to the DMDB subdirectory On the left panel you will see the database table. Choose GenBankProjects Click “rebuild”. Repeat for the GenBank Authors and GenBankReferences tables, if needed. PLEASE NOTE: I did this as listed and the rebuilding failed, but I went back and started the .exe file using the right click then Run as adminstrator like you have to do for DNA master. When I did that, the rebuilding worked. Dave |
| Link to this post | posted 24 Jun, 2020 21:17 | |
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I just ran into the same problem and forgot to check the forum but contacted Jeffrey Lawrence directly. I asked him what the BLOB error stood for and how to fix it. Here is his reply: >>>>> It means the database file was corrupted. Most times, this can be fixed. This is esoteric, but give it a try: Close DNA Master Go to the Helper directory in the DNA Master installation directory Look for a program called “dutil32.exe”. Run it. In the top box, click the “By Directory” button Navigate to the DNA master installation directory, then to the DMDB subdirectory On the left panel you will see the database table. Choose GenBankProjects Click “rebuild”. Repeat for the GenBank Authors and GenBankReferences tables, if needed. PLEASE NOTE: I did this as listed and the rebuilding failed, but I went back and started the .exe file using the right click then Run as adminstrator like you have to do for DNA master. When I did that, the rebuilding worked. Dave |