Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
fushigi_draft 52 homing endonuclease + primase
| Link to this post | posted 18 Jun, 2018 02:55 | |
|---|---|
|
Not sure exactly how to annotate this gene from fushigi (37,851-36310 rev) most A1 phage in this region have the two DNA primase genes with the large overlap, however in fushigi and a few others there is a single large gene of 513 amino acids: The first 128 amino acids have an HHPRED hit (98.8%)to DNA primase [PDB 5VAZ] amino acids 113 - 424 have HHPREd hit to INTEIN-ENCODED HOMING ENDONUCLEASE (99.9%) {PDB 1DQ3] and finally amino acids 414 - 513 also HHPRED (98.6%) the same DNA primase [PDB 5VAZ] most of the annotations in this pham are just DNA primase, one protein Turj99 gp53 is annotated (intein-containing topoisomerase primase). Clearly this is an intein within a primase, just not sure how best to annotate. |
| Link to this post | posted 18 Jun, 2018 12:12 | |
|---|---|
|
Cool! I wonder if we can use this one to figure out the boundaries of the others? I think DNA primase with intein is appropriate here. |
| Link to this post | posted 18 Jun, 2018 15:17 | |
|---|---|
|
|
I thought the same thing but no luck. The intein is entirely within the second (in order of transcription) gene and the upstream gene appears to no longer be expressed. |