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Recent Activity
Trimming tRNAs
| Link to this post | posted 29 Sep, 2024 15:27 | |
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Hi, It's been a very long time since my students or I had to trim a tRNA. I've looked on all the videos and on how to document the tRNA. On Aragorn and DNA Master it seems to be matching in start and stop codons as well as length, but the ends are different on the structure. I've circled it in red where I think it's different. How, do I fix this so that I can submit it for preliminary review? Thanks, |
165Kb| Link to this post | posted 30 Sep, 2024 00:08 | |
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Hi Sarah, tRNAs are called by Aragorn 1.1 in DNA Master. The ends were corrected in a later version. The on-line version calls the ends correctly (version 1.2.41) The best set of directions in the Bioinformatics Guide are found here: https://seaphagesbioinformatics.helpdocsonline.com/documenting-trnas You just enter the coordinated in the 3'5'fields of the feature table and copy/paste the product from the on-line version of Aragorn tRNA. I have circled where you enter the coordinates on your power point. debbie |
| Link to this post | posted 30 Sep, 2024 11:37 | |
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Thank you. |