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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Frameshift in a singleton, OnionKnight
| Link to this post | posted 12 Apr, 2023 17:36 | |
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My class and I are working on OnionKnight, a singleton that came from another institution. Genes 15/16 have all the appropriate features associated with being the tail assembly chaperone ORFs, but there's nothing to compare it with. Gene 15 runs from 9987 to 10592, and there's a conspicuous GGGAAA from 10484 to 10489, although it's followed not by a purine but by a C. I don't see anything else that screams "here's the frameshift." Is it safe to assign 10487 as the location of a -1 frameshift, or should I just skip it and leave the second ORF alone? |
| Link to this post | posted 13 Apr, 2023 00:57 | |
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Mitch, That is sufficient data to call the -1 frameshift. Best, debbie |
| Link to this post | posted 13 Apr, 2023 00:59 | |
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Thanks, Debbie! -Mitch |