Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look. So I made a picture! Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you ...
Archives week 43 of 2014
Oct. 27, 2014 - Nov. 2, 2014
3' versus 5' overhangs in sequencing data
Written by
on Oct. 31, 2014 .