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The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Archives Oct. 31, 2014

3' versus 5' overhangs in sequencing data

3' versus 5' overhangs in sequencing data

Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look.  So I made a picture!  Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you ...

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