SEA-PHAGES: BioS 118 Phage Genetics Lab

Primary Instructor: Vassie Ware

Additional Instructor: Margaret Kenna

Type: In silico (phage genomics/bioinformatics)

Hosts used: Mycobacterium smegmatis

Number of students: 10

Sophomores: 10

Meetings per week: 2

Hours per week: 5.5

Bioinformatics work: Annotation of two genomes from DOGEMS analyses Wet lab experiments also include: 1)PCR to determine which phages correspond to specific contigs in DOGEMS samples 2) preparation of lysogens using phages from in situ phage hunting course last semester 3) immunity testing of phages on newly made lysogens and other lysogens