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Recent Activity
All posts created by srherrle
| Link to this post | posted 18 Apr, 2018 18:31 | |
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When annotating phage Squiddly (DN2) two genes have evidence of being an integrase, (Y-int) - Gene 44 and Gene 38. Based on Blast hits, HHPred, and genomic position upstream of what I believe is the immunity repressor (gene 4 I have called gene 44 as the integrase; however, gene 38 also contains tyrosine XerC domains, has hits to integrases with 100%probability on HHPred and is in a Phamily that have called this protein the integrase in Phage Howe gene 36 and Bowser gene 37.Can there be multiple tyrosine integrases in a Gordonia phage genome? I'm not sure why the immunity repressor number is showing as a smiley face, but that should say forty eight. |
Posted in: Cluster DN Annotation Tips → Multiple Integrases?
| Link to this post | posted 18 Jan, 2017 15:36 | |
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We're having lots of students (but not all students) that continue to get the "Glimmer Failure" error even though they have updated DNA Master. As noted above, the majority of these students are the ones running DNA Master on Macs using WINE. |
Posted in: DNA Master → Glimmer Failure on Auto Annotation