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Multiple Integrases?
| Link to this post | posted 18 Apr, 2018 18:31 | |
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When annotating phage Squiddly (DN2) two genes have evidence of being an integrase, (Y-int) - Gene 44 and Gene 38. Based on Blast hits, HHPred, and genomic position upstream of what I believe is the immunity repressor (gene 4 I have called gene 44 as the integrase; however, gene 38 also contains tyrosine XerC domains, has hits to integrases with 100%probability on HHPred and is in a Phamily that have called this protein the integrase in Phage Howe gene 36 and Bowser gene 37.Can there be multiple tyrosine integrases in a Gordonia phage genome? I'm not sure why the immunity repressor number is showing as a smiley face, but that should say forty eight. |
| Link to this post | posted 19 Apr, 2018 14:51 | |
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Yes, looks like Squiddly has two integrases, including one of the same ones that appears in several other gordonias with two integrases: Howe, Utz, Bowser. And yes, you can have two tyrosine ones. We talk about it in the Gordonia paper: http://mbio.asm.org/content/8/4/e01069-17.short Good catch! |