Link to this post | posted 04 Oct, 2022 15:49
sbconant	Maybe? There are lots of lysogenic plaques so it makes it a little harder to tell. We will filter and replate again today and see if the same thing happens! All other plates made in the same batch are tan/not greenish. My first guess is some kind of contamination but it is weird that it is not in top agar and only turned green after plating with phage.

Link to this post | posted 04 Oct, 2022 15:21

sbconant

We have a new issue! For one student only, when purifying phage using M.smeg her agar on her plate is turning a light green. No other contamination is evident and no other students are having the same issue. Is there something about a particular phage that might cause the agar to turn green?? For clarification - not the top agar containing phage and smeg, only the plate agar from original pour. All other plates and top agar are fine (typical color). Thanks!

Link to this post | posted 03 Sep, 2015 00:56
sbconant	Yes of course, thank you! Stephanie

Link to this post | posted 02 Sep, 2015 15:55
sbconant	Is there any way someone might be able to send me a pdf of "The secret lives of mycobacteriophages"?? I cant seem to access it through our or Wayne State's library system. Thanks!

Link to this post | posted 01 Sep, 2015 16:56
sbconant	Thank you so much!

Link to this post | posted 01 Sep, 2015 15:56
sbconant	I remember several people mentioning good research or introductory phage articles that they have their students read at the intro to this course. I can't, however seem to find in my notes what they were! Any help would be greatly appreciated. Thanks, Stephanie