SEA-PHAGES | All posts created by rodney

Link to this post \| posted 07 Nov, 2016 15:37
rodney_king	Viknesh Sivanathan Hi Rodney, I can't say I've seen this, and have not yet heard from anyone with this problem. Perhaps someone who has will chime in. In the meanwhile, would you share a gel where you see the degradation only in the presence of restriction enzyme buffer? I assume the buffer isn't contaminated with DNAse, and that you are using brand new buffer when you used the new enzymes? Vic Hi Vic, I am happy to share gel images. I will post them soon. Yes, we have used brand new buffers and enzymes. We also tested the "old" enzyme and buffers on DNA isolated using the old protocol and on plasmid DNA using a different DNA isolation kit. These DNA samples cut normally and there was no sign of degradation. Rodney

Link to this post | posted 07 Nov, 2016 15:37

Viknesh Sivanathan
Hi Rodney,

I can't say I've seen this, and have not yet heard from anyone with this problem. Perhaps someone who has will chime in.
In the meanwhile, would you share a gel where you see the degradation only in the presence of restriction enzyme buffer? I assume the buffer isn't contaminated with DNAse, and that you are using brand new buffer when you used the new enzymes?

Vic

Hi Vic,
I am happy to share gel images. I will post them soon. Yes, we have used brand new buffers and enzymes. We also tested the "old" enzyme and buffers on DNA isolated using the old protocol and on plasmid DNA using a different DNA isolation kit. These DNA samples cut normally and there was no sign of degradation.
Rodney

Posted in: Phage Discovery/Isolation → Yield and degradation of DNA isolated by new protocol

Link to this post \| posted 03 Nov, 2016 14:54
rodney_king	Has anyone experienced degradation of DNA isolated by the new protocol? We tried a brand new DNA isolation kit and brand new enzymes and still see degradation. Never experienced this problem before. The fact that the DNA disappears only in the presence of restriction enzyme buffer indicates DNAse is not being thoroughly removed.

Posted in: Phage Discovery/Isolation → Yield and degradation of DNA isolated by new protocol

Link to this post \| posted 24 May, 2016 17:48
rodney_king	I was shocked and deeply saddened by this tragic news. David brought an unparalleled enthusiasm to the SEA program. Sincerest sympathies to his family and his colleagues at Cabrini.

Posted in: Messages for Cabrini College → Messages for Cabrini College

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