Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
All posts created by khafer
| Link to this post | posted 21 May, 2019 17:53 | |
|---|---|
|
additional photo |
579KbPosted in: Phage Biology → DNA Smear
| Link to this post | posted 21 May, 2019 17:52 | |
|---|---|
|
|
additional photos…can't figure out how to add multiple attachments! |
Posted in: Phage Biology → DNA Smear
| Link to this post | posted 21 May, 2019 17:50 | |
|---|---|
|
|
We had problems with smeary DNA for the first time this year. We used the host Streptomyces griseofuscus, which we also used last year without problems. Eventually I found that the uncut DNA looked fine if I ran it out right after isolation, but even one day in the freezer and the DNA looked smeary. Sorry I don't have gels directly showing this but I have some gels showing non-smeary and smeary DNA. We eventually changed DNA preps and sent the DNA we got for sequencing. The good news is all the samples worked fine for sequencing. Kathy |
Posted in: Phage Biology → DNA Smear