Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Debbie Jacobs-Sera posted in Whole phage starterator reports
cdshaffer posted in Whole phage starterator reports
Shallee Page posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
Debbie Jacobs-Sera posted in Plaques appear on spot test, don't appear in titer
All posts created by khafer
Link to this post | posted 21 May, 2019 17:53 | |
---|---|
|
additional photo |
Link to this post | posted 21 May, 2019 17:52 | |
---|---|
|
additional photos…can't figure out how to add multiple attachments! |
Link to this post | posted 21 May, 2019 17:50 | |
---|---|
|
We had problems with smeary DNA for the first time this year. We used the host Streptomyces griseofuscus, which we also used last year without problems. Eventually I found that the uncut DNA looked fine if I ran it out right after isolation, but even one day in the freezer and the DNA looked smeary. Sorry I don't have gels directly showing this but I have some gels showing non-smeary and smeary DNA. We eventually changed DNA preps and sent the DNA we got for sequencing. The good news is all the samples worked fine for sequencing. Kathy |