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Recent Activity
All posts created by kclifton
| Link to this post | posted 03 Jun, 2021 23:05 | |
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Thank you Debbie! I appreciate the info, this was very frustrating. At least happy to know it's not something I did/did not do. Kari |
Posted in: DNA Master → one gene won't blast
| Link to this post | posted 01 Jun, 2021 03:14 | |
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I am finishing an annotation for submission. We used PECAAN and I pulled in the data and parsed the file successfully. When I re-blasted the file, the BIG gene (Podoviridae, so not tape measure but hypothetical protein)will not blast. I get an error and no blast data for only this gene. I've tried blasting just the single gene. Error every time. BUT - when I first did the original blast, the gene blasted just fine. I have a new computer now, so if it's my computer I don't know what it might be. CPU usage is nowhere near maxed out. I checked my newer blasted genome for a different phage and the tape measure gene blasted just fine on this same computer, and it's a lager gene than the one I'm having trouble with. I checked the sequence, it's fine. I can copy and paste it from DNAMAster and blast directly and it works. PECAAN blasted it fine as well. So I tried an old file with no PECAAN data parsed into it, and it won't blast from that file either. I sent it to my colleague and she can't get any blast results for this gene, whether a single gene or whole genome blast. I'm going to bring a student laptop home tomorrow and try it on that. I don't know what else to do. This one thing is preventing me from submitting the annotation. Any suggestions are much appreciated. Kari |
Posted in: DNA Master → one gene won't blast