SEA-PHAGES | All posts created by jeremy

Link to this post \| posted 05 Sep, 2018 02:27
jeremy	So M. Smeg grown early in the semester will be fine to use even 3 months later? Two consecutive semesters (Fall2017/Spring201 we were not able to get plaques (first time we got 10/12). We requested fresh M. Smeg but were referred back to our original stocks. It was suggested that perhaps the bacteria had to be in a particular phase of growth and/or aerobic state to work but we still had issues with plaque formation after growing/using fresh cultures every 5 days. We are able to get some plaques now, and hopefully it works this Fall semester, but are not sure what the problem was/is. Anyway, I know the guide says once a culture is grown to saturation it should be good for "weeks to months", and we were able to successfully use a few weeks-old culture the first time. I've not worked with Mycobacteria before this and was curious if "old" bacteria could be a cause of any of our problems..? Thanks!

Link to this post | posted 05 Sep, 2018 02:27

So M. Smeg grown early in the semester will be fine to use even 3 months later?

Two consecutive semesters (Fall2017/Spring201 smile

we were not able to get plaques (first time we got 10/12). We requested fresh M. Smeg but were referred back to our original stocks. It was suggested that perhaps the bacteria had to be in a particular phase of growth and/or aerobic state to work but we still had issues with plaque formation after growing/using fresh cultures every 5 days. We are able to get some plaques now, and hopefully it works this Fall semester, but are not sure what the problem was/is.
Anyway, I know the guide says once a culture is grown to saturation it should be good for "weeks to months", and we were able to successfully use a few weeks-old culture the first time. I've not worked with Mycobacteria before this and was curious if "old" bacteria could be a cause of any of our problems..? Thanks!

Posted in: Phage Discovery/Isolation → Clumpy smeg

Link to this post \| posted 27 Feb, 2018 21:35
jeremy	Hello, I'm looking for a nice image of D29 digested by the following restriction enzymes: BamHI, ClaI, EcoRI, HaeIII, HindIII, SalI. I believe the samples used in the student guide (p7 is D29 but if anyone has additional images they would like to share please do. Thank you!

Posted in: Phage Biology → D29 RE Digest Image

Link to this post \| posted 05 Feb, 2018 18:07
jeremy	Is there a growth curve for M. Smeg available? Instructions say to grow for 3-5 days (until saturated)but also to have bacteria in the log phase of growth. From what I have seen this is around 30 hours and D29 does not seem to infect (on plates) then. Just looking for info outside of the instruction guide on using M. Smeg. Thanks Edited 05 Feb, 2018 18:11

Posted in: Mycobacterium → M. Smeg Growth Curve

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