SEA-PHAGES | All posts created by ejyoon

Link to this post \| posted 09 Feb, 2021 01:58
ejyoon	Hello, we are annotating Pham 51023 in Figliar, and we can't find a starterator report for this pham. This pham is not an orpham as there are 114 members in this pham. I looked up the pham in phagesDB and it is there, although I do not know how to tell if a pham number has been changed in an update or not. I was hoping to get help with obtaining the starterator report for this pham.

Posted in: Starterator → Pham not found in Starterator

Link to this post \| posted 10 Oct, 2020 16:26
ejyoon	Thank you for the reply, Debbie. The UV treatment method is working reasonably well for our lab this semester. Thank you for the suggestion about spotting one culture on the lawn of another. We'll keep that in mind for the future.

Posted in: Lysogeny/Immunity → Inducing phages from lysogeny to lytic cycle

Link to this post \| posted 21 Sep, 2020 17:13
ejyoon	Hello, We are seeking some advice about the best protocols or practices to induce and "force" bacteriophages to switch from a lysogenic cycle to a lytic cycle for the purification and amplification steps of phage discovery. We are using Gordonia rubripertincta for our host organism this fall. We are contemplating exposing the infected bacterial cells (right after being plated with top agar) to UV radiation for very short time periods. We recently tried growing the infected bacteria at a higher temperature than their optimal to force the phages out of lysogeny; however, that method did not work. Any suggestions and insight are appreciated. Thank you for your time. Edited 21 Sep, 2020 17:14

Link to this post | posted 21 Sep, 2020 17:13

ejyoon

Hello,

We are seeking some advice about the best protocols or practices to induce and "force" bacteriophages to switch from a lysogenic cycle to a lytic cycle for the purification and amplification steps of phage discovery. We are using Gordonia rubripertincta for our host organism this fall.

We are contemplating exposing the infected bacterial cells (right after being plated with top agar) to UV radiation for very short time periods. We recently tried growing the infected bacteria at a higher temperature than their optimal to force the phages out of lysogeny; however, that method did not work.

Any suggestions and insight are appreciated. Thank you for your time.

Edited 21 Sep, 2020 17:14

Posted in: Lysogeny/Immunity → Inducing phages from lysogeny to lytic cycle

Link to this post \| posted 23 May, 2019 05:03
ejyoon	We saw some smearing in our gels. This was our first year so we did not know what to expect and believed the smearing to be due to human or equipment error (and we are not ruling that out). I've attached an image of a gel that shows faint smearing throughout the cut lanes (not much uncut DNA present in the first lane unfortunately). Fortunately, we were able to get sequencing back for our phages (the attached gel image is from one of those sequenced phages). 257Kb

Link to this post | posted 23 May, 2019 05:03

ejyoon

We saw some smearing in our gels. This was our first year so we did not know what to expect and believed the smearing to be due to human or equipment error (and we are not ruling that out). I've attached an image of a gel that shows faint smearing throughout the cut lanes (not much uncut DNA present in the first lane unfortunately). Fortunately, we were able to get sequencing back for our phages (the attached gel image is from one of those sequenced phages).

Posted in: Phage Biology → DNA Smear

Recent Activity

All posts created by ejyoon