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c.sunnen posted in F1 cluster forward reverse or neither?

Debbie Jacobs-Sera posted in F1 cluster forward reverse or neither?

c.sunnen posted in F1 cluster forward reverse or neither?

c.sunnen posted in F1 cluster forward reverse or neither?

Debbie Jacobs-Sera posted in F1 cluster forward reverse or neither?

All posts created by cnoutsos

| posted 23 Jun, 2020 23:32
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cnoutsos
debbie
Do you a complete set of documentation in one of your files? Copy it from whatever corrupted file it is in and paste it into the (empty) documentation of a perfect sequence file. (To get that sequence file, open a .fasta and stop before you auto-annotate.) Then parse. You won't have blast data, but you also won't have to re-enter as much. You can cut and paste from multiple files. Sorry I can't do better.

Yes I understand. Thank you so much for your help and support !
Posted in: DNA Master → TbFeatures error
| posted 23 Jun, 2020 23:24
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cnoutsos
debbie
Christos,
I unpacked one of my files, so how your file was broken is extraordinary. My next advice is to revert to an older file that is not broke or to cut and paste from documentation. let me know if I can help.
debbie

When you say cut and past from documentation you mean to copy and paste the things I need to a fresh file that is not corrupted?

Thank you so much for your help!
Posted in: DNA Master → TbFeatures error
| posted 23 Jun, 2020 15:07
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cnoutsos
debbie
Christos,
I am still unsuccessful, but checking. Can you open and use a different .dnam5 file on your machine?
Was PECAAN used to create your file? What is the next best version of your file?
debbie

No PECAAN was not used. So this is what happens: When I first open the file features are empty. When I reopen it I get the features . If I save the file with a different name I still have the same issue.
Posted in: DNA Master → TbFeatures error
| posted 23 Jun, 2020 14:19
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cnoutsos
cnoutsos
Thank you Debbie, attached please find the file
Hi Debbie,
I was wondering if you had a chance to see the file . Any help will be highly appreciated.
Christos
Posted in: DNA Master → TbFeatures error
| posted 18 Jun, 2020 13:02
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cnoutsos
Thank you Debbie, attached please find the file
26Kb
Posted in: DNA Master → TbFeatures error
| posted 16 Jun, 2020 17:07
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cnoutsos
debbie
Hi Christos,
This how you fix the file. It is a bit tedious.
https://seaphagesbioinformatics.helpdocsonline.com/article-104
Good luck,
debbie

Hi Debbie,
my student tried and nothing really happened. Even the features that he auto annotated were erased and the same error when trying to open Frames occurred. Any suggestions?
Posted in: DNA Master → TbFeatures error
| posted 12 Jun, 2020 01:19
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cnoutsos
Hi a student working with me has the attached error. He is on the latest version of DNAMaster .Any help would be highly appreciated.
Thank you!
514Kb
Posted in: DNA Master → TbFeatures error
| posted 15 May, 2018 14:42
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cnoutsos
We have a similar but a bit more complicated case: A C1 genome that ends with a gene starting at 155863 ending at 155955 and a second gene starting at 155596 ending to position 18. However there is the 1st gene that starts at position 15..
Any ideas how to deal with that??
Posted in: Choosing Start Sites → How to choose the start of the first gene for a circularly permuted genome