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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
All posts created by clbarrett
| Link to this post | posted 16 Oct, 2024 12:59 | |
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And just like that, the phages have popped back up using a subculture from the frozen stock. We are back on track again! Thank you! |
Posted in: Arthrobacter → Arthrobacter vanishing plaques
| Link to this post | posted 11 Oct, 2024 13:57 | |
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Thanks, Debbie. I threw out all media and started fresh Monday, still same issue. Started new cultures from stock yesterday, they should be ready for next week's labs. In the meantime, I've started students on Gordonia terrae, which is cooperating. I'll work out the issues with the Arthrobacter and we can go back to it but we are running short on time! Thanks so much for your help! Colleen |
Posted in: Arthrobacter → Arthrobacter vanishing plaques
| Link to this post | posted 11 Oct, 2024 13:29 | |
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debbie There is no evidence of phage on a plate spotted with Liebe, the positive control. |
Posted in: Arthrobacter → Arthrobacter vanishing plaques
| Link to this post | posted 10 Oct, 2024 15:29 | |
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Completely forgot that I had it! I don't use it past day 1 to avoid students reisolating it. Will let you know tomorrow when the spot plate grows. Thanks, Debbie! Colleen |
Posted in: Arthrobacter → Arthrobacter vanishing plaques
| Link to this post | posted 10 Oct, 2024 14:19 | |
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Hi everyone, We've been working with A. globiformis this semester and have suddenly hit a standstill. For the past two weeks we have been unable to reisolate phage picked from spot plates. Students will plate a dilution series and a beautiful, dense lawn of Arthrobacter shows up, but no plaques. In addition, we have processed 60+ samples in the past few days resulting in zero phage. Any ideas greatly appreciated! |
Posted in: Arthrobacter → Arthrobacter vanishing plaques