Link to this post | posted 16 Sep, 2023 03:09
bgibb	Hi Tammy, We've started looking at holins, but not exhaustively. Its difficult to conclusively identify holins, but there are candidates located proximal to some of the endolysins in AZ, but not in others.

Link to this post | posted 07 Sep, 2023 20:04
bgibb	We're working to finish up a paper that describes these reference AZ phages and the subclusters breakdown for them, so no- not yet.

Link to this post | posted 03 Jun, 2023 14:32

bgibb

A working group of SEA faculty harmonized 33 AZ phages. These phages have had their start sites and functions checked and when necessary, corrected in genbank submissions. The following AZ phages were included in this process and should be used as reference when annotating new AZ phages. Adolin Adumb2043 Amyev Asa16 Cassia Crewmate DrManhattan DrSierra Elezi Emotion Eraser Janeemi JohnDoe Kaylissa KeAlii Lego Liebe Lizalica London Maureen Niobe ObiToo Phives Powerpuff Reedo Tbone Tuck Tweety19 VResidence VroomVroom Warda Yang YesChef

Link to this post | posted 03 Jun, 2023 14:27
bgibb	All AZ phages found so far have a tail assembly chaperone with a programmed translational frameshift. The tail assembly chaperone is typically found in the left arm of the genome around gp14-16.

Link to this post | posted 03 Jun, 2023 14:19
bgibb	AZ phages contain a single endolysin gene, but the location within the genome and the pham for the endolysin gene is not consistent across the entire cluster. At least three patterns are observed within the cluster. Example include: Phives - Endolysin as gp1 Elezi - Endolysin in middle of genome, gp25 Powerpuff - Endolysin in right arm of genome, gp59 Edited 06 Jun, 2023 02:29

Link to this post | posted 06 May, 2022 03:06

bgibb

You're correct– I was looking at the instructor guide instead of the discovery guide. Thanks! viknesh bgibb There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed? I've had good luck with the pelco tab method. Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc

Link to this post | posted 05 May, 2022 17:19
bgibb	There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed? I've had good luck with the pelco tab method.