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Recent Activity

All posts created by Thiel

Link to this post \| posted 22 Mar, 2019 18:36
Thiel	Hello, This is my first time running SEA-PHAGES and we have some 10^11 and 10^12 titers. I saw this post but I wasn't sure if we needed to dilute them first because I remember at a meeting someone mentioning that it needed to be diluted if the titer was really high. I can't find it in the protocol now but I remember it being talked about a lot. Suggestions? Just try it and see what happens or should I try diluting it? Thanks, Sarah

Link to this post | posted 22 Mar, 2019 18:36

Thiel

Hello,

This is my first time running SEA-PHAGES and we have some 10^11 and 10^12 titers. I saw this post but I wasn't sure if we needed to dilute them first because I remember at a meeting someone mentioning that it needed to be diluted if the titer was really high. I can't find it in the protocol now but I remember it being talked about a lot. Suggestions? Just try it and see what happens or should I try diluting it?

Thanks,
Sarah

Posted in: Phage Discovery/Isolation → new DNA protocol with really high titer lysates

Link to this post \| posted 21 Mar, 2019 14:08
Thiel	Debbie, This makes sense. Thank you! Sarah

Posted in: Host-Range Project → Basic Host Range Project Information

Link to this post \| posted 20 Mar, 2019 18:48
Thiel	Hello, I am interested in doing this project. Currently we are using M. foliorum. Would you suggest that I start with one additional host or several? Thank you, Sarah Edited 20 Mar, 2019 18:58

Posted in: Host-Range Project → Basic Host Range Project Information