Link to this post | posted 16 Dec, 2020 22:02
DrCatalase	Is it okay to have "hypothetical protein" in the function field of your your complete notes file? Thank you.

Link to this post | posted 15 Dec, 2020 22:01
DrCatalase	cdshaffer ok release of starterator reports for database version 387 are now available and are tagged as the current version. Links from pecaan and phagesdb should now be working (at least until the next database update). Please report if any links fail at this point. Thank you, Chris!

Link to this post | posted 15 Dec, 2020 17:06
DrCatalase	cdshaffer These are typically due to database at phagesdb being out of sync with the current database for the starterator reports but this should not be happening "for weeks". Are you using pecaan or phagesdb for your links? Could you post the pham numbers you are looking for? Used the links from both to doublecheck. Peel 30 pham 45194 Peel 33 pham 45744 Peel 32 pham 45569

Link to this post | posted 15 Dec, 2020 16:25

DrCatalase

PEEL K1 cluster phage has several genes (28, 30, and 31) with functions (30 and 31) that have been giving us the… Page/Report not found Error 404 The requested Pham report could not be found. Possible reasons for a missing pham report beyond a simply typo in the URL: 1. Your protein is an orpham (reports are not generated for phams with only one member as there is nothing to compare it to.) Check phagesdb.org for the size of the pham. 2. The phamerator database has been updated and the pham number has changed. You can double check for the correct pham number at phagesdb.org. If neither of the above apply and you believe a report is missing in error it is important that you please post to the seaphages starterator forum. Error for weeks. This is through Pecaan and Phagesdb. Thank you. Sean

Link to this post | posted 14 Nov, 2019 15:17
DrCatalase	Thank you all. I will try cleaning the DNA and digesting for longer. BTW I did the PEG protocol 9.2a from the instructor's guide. Edited 14 Nov, 2019 15:27

Link to this post | posted 13 Nov, 2019 22:10

DrCatalase

Help, We got decent yields of DNA that and we tried to digest with NspI. the lanes are from two different phage lysates. Lanes: 1. DNA1 water 2. DNA1 water Buffer NspI (NEB 37 degrees 15 minutes) 3. DNA1 water Buffer 4. Ladder 5. DNA2 water 6. DNA2 water Buffer NspI (NEB 37 degrees 15 minutes) 7. DNA2 water Buffer We got no digestion except smearing in lane 6… we want to try again anyone have any suggestions? Thanks, Sean 90Kb

Link to this post | posted 30 Apr, 2019 13:51
DrCatalase	After uranyl acetate staining how long is the sample on the grid usable for EM? Thanks Sean

Link to this post | posted 08 Dec, 2018 03:48
DrCatalase	Thanks I will try tomorrow.

Link to this post | posted 08 Dec, 2018 00:54
DrCatalase	In the pane that lists all of the ORFs etc there is only one thing listed. I should have taken a picture.

Link to this post | posted 07 Dec, 2018 23:40
DrCatalase	Trying to set up DNA Master on Wine for the upcoming Bioinformatics workshop and after I hit auto annotate I only get one sequence in the features tab of the Extracted from FASTA Library Shubert.FASTA window. Please help.