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a gene in this gap?

| posted today, 13:18
Kissaou from Austin Community college wrote:
I am kissaou , and I am making this post regarind Mycobacterium phage "Teodoridan" Cluster A1
we completed the annotation, but I am a little it not happy about the 311 bp between gene 2 and gene 3.
Any idea? Looking at DNA master frames, it is possible to inser a gene between Start 1474bp - stop 1665bp. However, looking at glimmer and genemarks, there is not a reall coding potential.
What everybody else think?
Thanks
Kissaou
| posted today, 13:23
Kissaou,
The power of comparative genomics will aid in this quest to see if it is plausible that a gene is present in this area.
If there an open reading frame that looks plausible. you can blast n and blast p the area.
if this area is highly conserved, what are the possible explanations? If it is not conserved, what does that tell you. Check out the dates of the genomes that have this sequence. What have they called. What is the evidence on which they made their calls. That data is all present in the tools that you have access to. Lots of information, tricky decision.
let us know what you find out.
debbie
| posted today, 13:41
Thanks Debbie,
I will work on that and make a decision.
Thanks
Kissaou
 
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