Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
cdshaffer posted in Alphafold models of minor tail proteins
pchien posted in Alphafold models of minor tail proteins
pchien posted in Alphafold models of minor tail proteins
Debbie Jacobs-Sera posted in Adding genes in right arm of AS2 genomes
seaphages_usc posted in Adding genes in right arm of AS2 genomes
head-to-tail stopper
| Link to this post | posted 24 Mar, 2023 16:35 | |
|---|---|
|
I’m currently working on phage Culver in the CQ1 cluster and am having an issue with calling the function of gene 37 (15874-16227). Significant BLASTp matches show a mix of head closure proteins (not listed on official function list), hypothetical proteins, and head-to-tail stopper proteins. HHPred populates multiple significant hits, one of which aligns to SPP1 gp16. In the official functions list notes for head-to-tail stopper we much “must have an HHPRED alignment to one of the following crystal structures: SPP1 16 (5A21 chain E or F in the macromolecular complex”. We do not have that match, however we do have a match with 7ZAW_1 Head completion protein gp16 of Bacteriophage SPP1, Head completion proteins, Connector Compex. Based on this, can we call this “head-to-tail stopper”? |
| Link to this post | posted 29 Mar, 2023 12:54 | |
|---|---|
|
I am willing to call this a head-to-tail stopper because the first hit is to gp20 of L5. while we have not updated the label of that gene, when you HHpred it, it is a good hit to the 5A21_G project, which is the gene that we are assigning the function had-to-tail stopper. debbie |