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260/280 ratios too high from DNA extraction

Link to this post \| posted 19 Jul, 2022 18:18
MSMC	We are using the Wizard DNA Clean-Up System kit for DNA extraction (as we have for many years), and recently our DNA samples have had extremely high 260/280 ratios (most between 2.6 to 3.0). Things we've tried: 1. New Kit (Guanididium and Columns) 2. New Water Eluent 3. New EDTA 4. New SDS 5. Syringe vs. Vacuum manifold Our spec seems fine, as other samples give normal ratios. The A260 seems high (vs. low 280), as when we run samples on the gel, the mass appears lower on the gel than calculated by the spec. We're running out of ideas, any thoughts?

Link to this post | posted 19 Jul, 2022 18:18

MSMC

We are using the Wizard DNA Clean-Up System kit for DNA extraction (as we have for many years), and recently our DNA samples have had extremely high 260/280 ratios (most between 2.6 to 3.0). Things we've tried:

1. New Kit (Guanididium and Columns)
2. New Water Eluent
3. New EDTA
4. New SDS
5. Syringe vs. Vacuum manifold

Our spec seems fine, as other samples give normal ratios. The A260 seems high (vs. low 280), as when we run samples on the gel, the mass appears lower on the gel than calculated by the spec.

We're running out of ideas, any thoughts?