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HHPred, Blast, and CDD Parameters
Link to this post | posted 07 Jul, 2022 23:46 | |
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Have you wondered what the settings for HHPred, NCBI-blastp, and CDD are used by PECAAN? The parameters that we run for HHPred are as follows: Hhsuitedb: "mmcif70/pdb70 NCBI_CD/NCBI_CD scope70/scope70 pfama/pfama", Proteomes: "", MsaGenMethod: "UniRef30", MsaGenMaxIter: "3", HhpredInclEval: "1e-3", MinSeqidQuery: "0", MinCov: "20", SSScoring: "2", AlignMacMode: "loc", MacThreshold: "0.3", Desc: "250", Pmin: "20", and Blastp from ncbi blast 2.13.0+ is ran remotely with -db nr -gapopen 11 -gapextend 1 -word_size 6 -threshold 21 -window_size 40 -evalue 0.05 -max_target_seqs 100 -outfmt 5 -remote and The CDD database is searched by rpsblast from ncbi blast 2.13.0+ with the following arguments -num_alignments=50 -evalue 0.01 7/7/2022 |