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JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
Serine Integrase
Link to this post | posted 03 Dec, 2021 18:48 | |
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Unlike most of the Microbacterium phages we have found to date (12-2-21), Cluster EH phages are temperate and do have a serine integrase. We see 3 different phams of serine integrases in these genomes so far. There are 7 members in this cluster at this time - Floof, GardenState, Gretchen, Honk, IAmGroot, Percival and Zeta1847. Their starts are called correctly. Look for the serine residue ~ 8-20 bases of the start. Serine integrases are sometimes called large recombinases because of their size. A good read is found here: https://pubmed.ncbi.nlm.nih.gov/26350324/ |