Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Debbie Jacobs-Sera posted in Help with Annotating Direct Terminal Repeats
Marie Fogarty posted in Help with Annotating Direct Terminal Repeats
cdshaffer posted in Help with Annotating Direct Terminal Repeats
Marie Fogarty posted in Help with Annotating Direct Terminal Repeats
Debbie Jacobs-Sera posted in Potential minor tail proteins in cluster GK
Blast error
| Link to this post | posted 03 Sep, 2020 14:59 | |
|---|---|
|
All, I am getting the following error when I try to blast a gene in DNAM on my PC running W10 (not a surface). Error connecting with SSL. error: 1409442E:SSL routines::SSL3_READ_BYTES:tlsv1 alert protocol version. Anyone else having any trouble or aware of anything going on? Or any ideas? Steve |
| Link to this post | posted 03 Sep, 2020 15:14 | |
|---|---|
|
|
Tried it several times with different files and it never works. I have seen another error message, though: Error connect with SSL. EOF was observed that violates the protocol. Which sounds really unfortunate, if you ask me. Steve |
| Link to this post | posted 03 Sep, 2020 16:35 | |
|---|---|
|
Hi Steve, You are just having a heck of time. Have you checked your DNA Master BLAST preferences? debbie |
| Link to this post | posted 03 Sep, 2020 16:41 | |
|---|---|
|
|
I hope they are correct, although you never know. But I just got to campus and tried it from a computer that I know DNAM works on, and hasn't been changed. Same error. Steve |
| Link to this post | posted 03 Sep, 2020 16:51 | |
|---|---|
|
|
Steve, I just got the same error. I'll look into it from my end. debbie |
| Link to this post | posted 03 Sep, 2020 16:58 | |
|---|---|
|
|
Thanks, guess it's more than just me, then. Steve |
| Link to this post | posted 03 Sep, 2020 19:02 | |
|---|---|
|
Debbie & Steve, I am also getting Error connecting with SSL. EOF was observed that violates the protocol. Roy |
| Link to this post | posted 03 Sep, 2020 21:30 | |
|---|---|
|
Hello, I'm trying to auto-annotate my phage that we got (before my class has to do it) and I pre-selected a few and it seems as though it isn't working. My progress has been at 0% for the past 3 hours and I only pre-selected 5 genes. It worked before with Ellie so I'm not sure why it's not working this time. Thanks! Jasmin |
| Link to this post | posted 04 Sep, 2020 00:22 | |
|---|---|
|
|
Hey again, I tried to blast just one gene and I also got Error connecting with SSL Jasmin |
| Link to this post | posted 04 Sep, 2020 12:30 | |
|---|---|
|
|
This is bad timing since I have 120 students blasting genes starting this weekend. Just to confirm, I checked it this morning on another computer and on the UMBC virtual desktop and got the same result. So it looks like either NCBI is having a problem or the connection between DNAM and NCBI has one. I did check NCBI online, and it is working, so it isn't the main BLAST system. Steve |