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Blast error

Link to this post \| posted 03 Sep, 2020 14:59
scaruso	All, I am getting the following error when I try to blast a gene in DNAM on my PC running W10 (not a surface). Error connecting with SSL. error: 1409442E:SSL routines::SSL3_READ_BYTES:tlsv1 alert protocol version. Anyone else having any trouble or aware of anything going on? Or any ideas? Steve

Link to this post \| posted 03 Sep, 2020 15:14
scaruso	Tried it several times with different files and it never works. I have seen another error message, though: Error connect with SSL. EOF was observed that violates the protocol. Which sounds really unfortunate, if you ask me. Steve

Link to this post \| posted 03 Sep, 2020 16:35
debbie	Hi Steve, You are just having a heck of time. Have you checked your DNA Master BLAST preferences? debbie

Link to this post \| posted 03 Sep, 2020 16:41
scaruso	I hope they are correct, although you never know. But I just got to campus and tried it from a computer that I know DNAM works on, and hasn't been changed. Same error. Steve

Link to this post \| posted 03 Sep, 2020 16:51
debbie	Steve, I just got the same error. I'll look into it from my end. debbie

Link to this post \| posted 03 Sep, 2020 16:58
scaruso	Thanks, guess it's more than just me, then. Steve

Link to this post \| posted 03 Sep, 2020 19:02
coomansr	Debbie & Steve, I am also getting Error connecting with SSL. EOF was observed that violates the protocol. Roy

Link to this post \| posted 03 Sep, 2020 21:30
JChahal	Hello, I'm trying to auto-annotate my phage that we got (before my class has to do it) and I pre-selected a few and it seems as though it isn't working. My progress has been at 0% for the past 3 hours and I only pre-selected 5 genes. It worked before with Ellie so I'm not sure why it's not working this time. Thanks! Jasmin

Link to this post \| posted 04 Sep, 2020 00:22
JChahal	Hey again, I tried to blast just one gene and I also got Error connecting with SSL Jasmin

Link to this post \| posted 04 Sep, 2020 12:30
scaruso	This is bad timing since I have 120 students blasting genes starting this weekend. Just to confirm, I checked it this morning on another computer and on the UMBC virtual desktop and got the same result. So it looks like either NCBI is having a problem or the connection between DNAM and NCBI has one. I did check NCBI online, and it is working, so it isn't the main BLAST system. Steve

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