It seems we keep hitting the PE/PPE proteins that are only and easily found in the Mycobacteria. Rather than avoid calling them because they can be part of functions that are not yet identified, let's start adding PE/PPE family proteins to the annotations. The attached document provides useful information to what to look for when deciding if your protein of interest has all of the necessary parts. It also includes a citation for a paper for reference. A good example is Kayacho_gene 88 (Cluster B6). However, these proteins are found in lots of phage genomes, so not a cluster specific topic.
Edited 28 Jul, 2020 21:37