Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Dan Russell posted in Congrats to Steve Caruso and Beth Wilkes — 2025 ASM Outstanding Instructor Award, Honorable Mention
ACMPhageHunters posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
cdshaffer posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
space for a promoter when changing direction?
| Link to this post | posted 05 Sep, 2019 19:30 | |
|---|---|
|
We are annotating a B Cluster phage with no subcluster. We have a reverse gene whose start site we'd like to call (to capture all CP) doesn't leave room for a promoter even though it is a reverse gene in a sea of forwards (it is a ~25 bp overlap). Other similar phages also leave no room for a promoter in this region of the genome. I'd love to get some of your thoughts on calling a start to include all CP but leave no room for a change of direction promoter. |
| Link to this post | posted 15 Oct, 2019 15:42 | |
|---|---|
|
while not the usual case, there are a few genes through the Actinobacteriophages that are oriented as you describe. So if it is conserved throughout the cluster, go ahead and call it. |