SEA-PHAGES | BE1 tRNAs

Link to this post \| posted 24 May, 2019 18:04
scaruso	I am checking a BE1 submission, which have 40+ tRNAs. I noticed, as I go though them that I would not have accepted all of these. I've already found four with acceptor stems of 5 bp, 6 bp, and 8 bp, and a couple with very low scores (10 and 14), but that are present in other BE1 phages. Before I deleted them, I wanted to make sure there was no reason these were in there outside the guidlines. Thanks! Steve Edited 24 May, 2019 18:08

Link to this post | posted 24 May, 2019 18:04

I am checking a BE1 submission, which have 40+ tRNAs. I noticed, as I go though them that I would not have accepted all of these. I've already found four with acceptor stems of 5 bp, 6 bp, and 8 bp, and a couple with very low scores (10 and 14), but that are present in other BE1 phages.

Before I deleted them, I wanted to make sure there was no reason these were in there outside the guidlines.

Thanks!

Steve

Edited 24 May, 2019 18:08

Link to this post \| posted 24 May, 2019 21:03
lhughes	Steve, I would just note that any tRNA calls for most of the BE1 phages already in GenBank were called using the rules AS THEY EXISTED AT THE TIME of their annotation. That could be part of the discrepancy. Keep in mind that some rules also overrule other rules, so that could also be part of the difference. Lee

Link to this post \| posted 24 May, 2019 21:12
scaruso	Ah. Thanks. That helps. Steve Edited 24 May, 2019 21:13

Link to this post \| posted 24 May, 2019 21:16
scaruso	These are big phages. S.

Link to this post \| posted 26 May, 2019 19:47
lhughes	Steven Caruso These are big phages. S. Yep! At least the large terminal repeats actually bring the total down a little bit (since both ends should be identical, you only have to annotate one end and then just match the other one), but still large. And the massive number of tRNA's increases the workload too. Lee

Recent Activity

BE1 tRNAs