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RBS Data
| Link to this post | posted 23 Apr, 2019 22:13 | |
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I have two questions about the RBS data that we are going to fill in our annotation, the guide says that we must write [NA/Scoring Matrix, spacing matrix, Z score, Final score, yes/no](1), and the way we used to calculate the RBS (2). 1) Is necessary all that information, because at the time to write it could be a little bit confusing and also, what does yes/no are the for? 2) I thought that we were going to be able to find this information in DNA Master –> ORG –> RBS I am working with phage Tubs. Thanks!
JM
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| Link to this post | posted 25 Apr, 2019 22:25 | |
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It is OK to indicate on your coversheet which scoring matrix and spacing matrix you used, as long as you don't change it on a per gene basis. Z-score and final score should still be included with every gene. Yes/no indicates if it is the best score or not, as compared to the other choices for that gene. |