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dihydrofolate reductase vs thymidylate synthase

Link to this post \| posted 25 Feb, 2019 23:24
debbie	This refers specifically to BubbaBear_gp56. A question posed by Dr. Herren (Kansas State U): Isn't this a dihydrofolate reductase rather than a thymidylate synthase. First I scanned this website http://www.ebi.ac.uk/interpro/entry/IPR012262 From this I learned that they often come together. However, when both parts are there, I think it is a much larger protein, with PDB citing residues of 159-384 for the DR crystal structure and 1042 – 2084 residues for the TS structure. It looks like this is aligning with the DR domains and not the TS. So I would call BubbaBear gp 56 a "dihydrofolate reductase"!

Link to this post | posted 25 Feb, 2019 23:24

debbie

This refers specifically to BubbaBear_gp56. A question posed by Dr. Herren (Kansas State U): Isn't this a dihydrofolate reductase rather than a thymidylate synthase.

First I scanned this website http://www.ebi.ac.uk/interpro/entry/IPR012262
From this I learned that they often come together.
However, when both parts are there, I think it is a much larger protein, with PDB citing residues of 159-384 for the DR crystal structure and 1042 – 2084 residues for the TS structure.
It looks like this is aligning with the DR domains and not the TS. So I would call BubbaBear gp 56 a "dihydrofolate reductase"!