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JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
Waleliano (B4) Functions (2875-3465 & 3458-5206)
Link to this post | posted 31 Oct, 2018 20:27 | |
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I am looking at 2 genes in Waleliano (B4) and trying to determine the correct functions. They are gp4 (2875-3465) and gp5 (3458-5206). I feel like the functions should be either terminase, small subunit and terminase, large subunit or polynucleotide kinase and terminase. My evidence for each case is below. Help appreciated! 2875-3465 F: terminase, small subunit SIF-BLAST: terminase, small subunit, phagesDB and NCBI, BrownCNA gp4, AKY02717.1, 100%, e-111 SIF-HHPred: no terminase matches SIF-Syn: terminase small subunit, downstream "terminase" and portal protein,BrownCNA and Hangman F: polynucleotide kinase SIF-BLAST: adenylate kinase, phagesDB and NCBI, PhrankReynolds gp1, AWN05584.2, 50%, 7e-42 SIF-HHPred: polynucleotide kinase, HHPred, Bacteriophage T4, d1ly1a_, %78.1, 99.32% SIF-Syn: NKF 3458-5206 F: terminase large subunit SIF-BLAST: terminase large subunit, phagesDB and NCBI, Hangman gp5, AXH47369.1, 99%, e=0.0 SIF-HHPred: terminase large subunit, HHPred, bacteriophage T4, 3CPE_A, 86.3%, 100 SIF-Syn: terminase large subunit, downstream portal protein, BrownCNA and Hangman F: terminase SIF-BLAST: terminase, phagesDB and NCBI, ChrisnMich gp4, AEJ94575.1, 98%, e=0 SIF-HHPred: phage terminase, HHPred, PF03354.14, 76.7%, 99.6% SIF-Syn: terminase, downstream portal protein, BrownCNA and Hangman |
Link to this post | posted 31 Oct, 2018 21:11 | |
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Kayla, I have been looking closely at the Cluster B1 phages. I concur with the genes being polynucleotide kinase and terminase. There is no evidence that a small terminase is present in the genome……yet. debbie |
Link to this post | posted 01 Nov, 2018 15:31 | |
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Debbie Jacobs-Sera Thanks! |