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frameshift
| Link to this post | posted 21 May, 2018 20:13 | |
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We think we have a translational frameshift of the tail assembly protein. We found the slippery sequence GGGAAA and annotated the two regions. When validating, we get a message saying the two genes share a 5' coordinate. Are we supposed to delete the first gene after editing the second gene to have 2 regions? this gets rid of the error, but I don't see that in the instructions. |
| Link to this post | posted 22 May, 2018 00:07 | |
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That message is a warning not an error. It comes up because it is easy to get duplicate genes if you use the DNA Master tool to merge multiple DNA Master files. In this case however, you have two genes, a short form and a long form of the TAC with the same 5' end and this is exactly what you want. So it is OK to ignore the warning. |
| Link to this post | posted 22 May, 2018 01:10 | |
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Awesome. Thanks chris |