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cdshaffer posted in Whole phage starterator reports
fbaliraine posted in Phage DoRead Start at 52714 bp with 8 bp overlap vs start at 52718 with 4 bp overlap?
Debbie Jacobs-Sera posted in Phage DoRead Start at 52714 bp with 8 bp overlap vs start at 52718 with 4 bp overlap?
Re-BLAST final dnam5 file
| Link to this post | posted 10 May, 2018 19:24 | |
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Hi - My question is about the request to re-BLAST all features before submitting your final DNA Master files for review. We have done that for all genes in which the start site was altered as well as for all added genes. But we did not re-BLAST all genes that were called using the original auto-annotated coordinates. Is that acceptable or do you require a complete re-BLAST be done? Thanks, Joe |
| Link to this post | posted 11 May, 2018 12:25 | |
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Hi Joe, As long as the BLAST data matches the start chosen in the file, you are all set– no need to reBLAST. Thanks for checking! |