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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
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nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Contamination of Wintermute from the Host Range Lysate Box
| Link to this post | posted 11 Sep, 2017 19:31 | |
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Recently, it was brought to my attention that the Wintermute lysate is contaminated. Upon investigation, it has been determined that Wintermute IS contaminated with Fionbharth. My apologies. Teachable moment: Whenever you receive a lysate, please always do the following: 1. Freeze the lysate for long term use. 2. Make a high titer lysate for your own use. Use preferred microbiology practice and make your phage lysate from a single plaque. PCR verify that plaque and your lysate. 3. Freeze aliquots of your high titer lysate for future use. debbie |