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Recent Activity
Patch test experiment
| Link to this post | posted 10 Apr, 2017 22:08 | |
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Some of our students are investigating potential lysogens. We have 2 questions: 1. Do we need to use 7H10 plates for the patch assay lysogen only plate, or can we use L-Agar plates without top agar? (if we do need the 7H10 plates, why is that?) 2. Can use use a wire loop rather than disposable loops? Thanks, Susan |
| Link to this post | posted 11 Apr, 2017 15:14 | |
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Susan, You can do the patch test on L-agar. Sure, why not! I don't understand the without top agar. the patch test is done to visualize phage release from a colony. So you need wild type smeg in the top agar for that detection. You make a companion plate with the patch test, so that your can carry that colony forward (without picking up WT smeg. does that make sense? You can always use a wire loop. Depending on numbers, that is a lot of firing the loop. You can also use sterile toothpicks for some of this testing. |
| Link to this post | posted 11 Apr, 2017 23:32 | |
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Thank you Debbie, that makes sense! Susan |