Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Debbie Jacobs-Sera posted in Help with Annotating Direct Terminal Repeats
Marie Fogarty posted in Help with Annotating Direct Terminal Repeats
cdshaffer posted in Help with Annotating Direct Terminal Repeats
Marie Fogarty posted in Help with Annotating Direct Terminal Repeats
Debbie Jacobs-Sera posted in Potential minor tail proteins in cluster GK
Adding Illumina reads
| Link to this post | posted 07 Nov, 2015 21:08 | |
|---|---|
|
I have tried to add Illumina reads to an assembly using "addSolexaReads.perl" and following Dan's instructions. However I keep getting the following error "-ace must be specified". Everything looks correct to me with the command and filenames. Any suggestions? Lee |
| Link to this post | posted 08 Nov, 2015 03:03 | |
|---|---|
|
|
Figured it out! I was using the old form, not the newer form of the command (forgot to read the comments at the bottom of the video). |
| Link to this post | posted 10 Nov, 2015 18:30 | |
|---|---|
|
Yep, you got it Lee. I made the original video with an older version of consed. The new syntax should be:–Dan |
| Link to this post | posted 23 Sep, 2023 19:12 | |
|---|---|
|
Hi Dan and Lee, I'm having the same question. I tried executing the new command you posted modified to my case: addSolexaReads.perl -ace 454Contigs.ace.1.ace -fastqfof Reads.fof -fasta 454Contigs.ace.1.contigs.fof Since my files are called 454Contigs.ace.1.ace and 454Contigs.ace.1.contigs and Reads.fof. But it still wouldn't work, including different variations I tried. Are there any key preceding steps that I should know that are not in your tutorials? I couldn't find that video with the comments at the bottom that you mentioned. Thanks very much. |