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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
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nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
hydrolase, esterase or hydrolase/esterase?
Link to this post | posted 12 Apr, 2016 22:13 | |
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Which is the preferred function annotation? TiroTheta9_59, hydrolase, 274 537 e-153 Sabertooth_60, esterase, 274 537 e-153 Melvin_MELVIN_62, hydrolase/esterase, 274 537 e-153 |
Link to this post | posted 13 Apr, 2016 19:21 | |
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Hi Roy, What a great question! Do you have HHPred or BLAST results that support the intact catalytic domain of an esterase? If so, go with esterase. Otherwise, more general might be better. Thanks! Welkin |
Link to this post | posted 13 Apr, 2016 20:20 | |
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Welkin, Here's my top hit on HHpred. I'm thinking the answer is yes, but I'm not sure how to interpret HHpred results in detail. >1zoi_A Esterase; alpha/beta hydrolase fold; 1.60A {Pseudomonas putida} SCOP: c.69.1.12 PDB: 4dgq _A Probab=100.00 E-value=2.3e-38 Score=242.53 Aligned_cols=255 Identities=24% Similarity=0.255 Sum_probs=0.0 |