While annotating the phage PhancyPhin, we came across a gene (#13) which we decided to delete as it was in the reverse orientation compared to all the others in that region, was about 107 bp long and had no coding potential. Now there is a gap of 116 bp between 12 and 14. The region is too small to insert a gene and also there are no suitable start and stop co ordinates. We see a similar gap in other members such as michellemybelle. So shall we let the gap be as it is? Gene 34 is another gene that when deleted with similar reasoning as above, leaves a gap of 206 bp, with no suitable start and stop co ordinates? So, is it alright to have a gap of 200 bp?
Thank you