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Two methionines at the N-terminus?
| Link to this post | posted 28 Mar, 2022 21:31 | |
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Greetings, all! If you have a choice of a start that provides a single initial Met, or a start three bases earlier that would result in the translation of a protein with two methionines at the N-terminus, is there a rule regarding which start site is the correct one? Looking at the BLAST data for this particular gene, some annotators went with one Met and others went with two. Thanks in advance for the help! Kathleen |
| Link to this post | posted 29 Mar, 2022 12:37 | |
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Kathleen, Yep. It is rule based on a small data set from some mass spec data. Choose the second one. debbie |
| Link to this post | posted 09 Jun, 2023 18:15 | |
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Talking to Debbie in person! We clarified that the start site is the second methionine in the sequence, which means that the translated protein has a single initial methionine and not two Mets, irrespective of the direction of translation. |