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genes 19/20 in K1 subcluster

Link to this post \| posted 02 Mar, 2021 21:06
susannepfeifer	Dear all, We are currently annotating the genome of Stinson, a cluster K1 phage. Looking at the Phamerator maps of several previously annotated K1 phages, it seems that genes 19 and 20 are annotated "on top of each other" which seems odd (and I vaguely remember Welkin mentioning in one of the tutorial videos that this might be a sign that something is wrong with the annotation). Any insights would be highly appreciated! Best, Susanne 363Kb

Link to this post | posted 02 Mar, 2021 21:06

susannepfeifer

Dear all,

We are currently annotating the genome of Stinson, a cluster K1 phage. Looking at the Phamerator maps of several previously annotated K1 phages, it seems that genes 19 and 20 are annotated "on top of each other" which seems odd (and I vaguely remember Welkin mentioning in one of the tutorial videos that this might be a sign that something is wrong with the annotation). Any insights would be highly appreciated!

Best,
Susanne

Link to this post \| posted 02 Mar, 2021 22:19
debbie	Hi Susanne, Yes. That is a classic presentation of a programmed frameshift found in tail assembly chaperone genes. There is some introductory info in the Bioinformatic Guide: https://seaphagesbioinformatics.helpdocsonline.com/article-54 https://seaphagesbioinformatics.helpdocsonline.com/article-6 Lots have been written about them in the literature. debbie

Link to this post \| posted 02 Mar, 2021 22:20
susannepfeifer	Thank you so much, Debbie!