SEA-PHAGES | Waleliano (B4) Functions (2875-3465 & 3458-5206)

Link to this post \| posted 31 Oct, 2018 20:27
kaylafast	I am looking at 2 genes in Waleliano (B4) and trying to determine the correct functions. They are gp4 (2875-3465) and gp5 (3458-5206). I feel like the functions should be either terminase, small subunit and terminase, large subunit or polynucleotide kinase and terminase. My evidence for each case is below. Help appreciated! 2875-3465 F: terminase, small subunit SIF-BLAST: terminase, small subunit, phagesDB and NCBI, BrownCNA gp4, AKY02717.1, 100%, e-111 SIF-HHPred: no terminase matches SIF-Syn: terminase small subunit, downstream "terminase" and portal protein,BrownCNA and Hangman F: polynucleotide kinase SIF-BLAST: adenylate kinase, phagesDB and NCBI, PhrankReynolds gp1, AWN05584.2, 50%, 7e-42 SIF-HHPred: polynucleotide kinase, HHPred, Bacteriophage T4, d1ly1a_, %78.1, 99.32% SIF-Syn: NKF 3458-5206 F: terminase large subunit SIF-BLAST: terminase large subunit, phagesDB and NCBI, Hangman gp5, AXH47369.1, 99%, e=0.0 SIF-HHPred: terminase large subunit, HHPred, bacteriophage T4, 3CPE_A, 86.3%, 100 SIF-Syn: terminase large subunit, downstream portal protein, BrownCNA and Hangman F: terminase SIF-BLAST: terminase, phagesDB and NCBI, ChrisnMich gp4, AEJ94575.1, 98%, e=0 SIF-HHPred: phage terminase, HHPred, PF03354.14, 76.7%, 99.6% SIF-Syn: terminase, downstream portal protein, BrownCNA and Hangman

Link to this post | posted 31 Oct, 2018 20:27

I am looking at 2 genes in Waleliano (B4) and trying to determine the correct functions. They are gp4 (2875-3465) and gp5 (3458-5206). I feel like the functions should be either
terminase, small subunit and terminase, large subunit
or
polynucleotide kinase and terminase.

My evidence for each case is below. Help appreciated!

2875-3465
F: terminase, small subunit
SIF-BLAST: terminase, small subunit, phagesDB and NCBI, BrownCNA gp4, AKY02717.1, 100%, e-111 SIF-HHPred: no terminase matches
SIF-Syn: terminase small subunit, downstream "terminase" and portal protein,BrownCNA and Hangman

F: polynucleotide kinase
SIF-BLAST: adenylate kinase, phagesDB and NCBI, PhrankReynolds gp1, AWN05584.2, 50%, 7e-42
SIF-HHPred: polynucleotide kinase, HHPred, Bacteriophage T4, d1ly1a_, %78.1, 99.32%
SIF-Syn: NKF

3458-5206
F: terminase large subunit
SIF-BLAST: terminase large subunit, phagesDB and NCBI, Hangman gp5, AXH47369.1, 99%, e=0.0 SIF-HHPred: terminase large subunit, HHPred, bacteriophage T4, 3CPE_A, 86.3%, 100
SIF-Syn: terminase large subunit, downstream portal protein, BrownCNA and Hangman

F: terminase
SIF-BLAST: terminase, phagesDB and NCBI, ChrisnMich gp4, AEJ94575.1, 98%, e=0
SIF-HHPred: phage terminase, HHPred, PF03354.14, 76.7%, 99.6%
SIF-Syn: terminase, downstream portal protein, BrownCNA and Hangman

Link to this post \| posted 31 Oct, 2018 21:11
debbie	Kayla, I have been looking closely at the Cluster B1 phages. I concur with the genes being polynucleotide kinase and terminase. There is no evidence that a small terminase is present in the genome……yet. debbie

Link to this post \| posted 01 Nov, 2018 15:31
kaylafast	Debbie Jacobs-Sera Kayla, I have been looking closely at the Cluster B1 phages. I concur with the genes being polynucleotide kinase and terminase. There is no evidence that a small terminase is present in the genome……yet. debbie Thanks!

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Waleliano (B4) Functions (2875-3465 & 3458-5206)