SEA-PHAGES | D29 plaques barely visible on M. smeg

Link to this post \| posted 27 Aug, 2018 18:56
debbie	Kayla, Would you do dilutions of D29 and not just a single spot? You streak from P1FF streak from stock #2 looks like smeg. When you see 4 day colonies, are they dry and wrinkled? (as in, not smooth and glistening?)

Link to this post \| posted 28 Aug, 2018 20:51
kaylafast	Viknesh Sivanathan kaylafast P2FF streak from stock #1 Incubation: 37 degrees C, 2 days (1 day photo unavailable) I would be suspicious of this streak plate (and the culture from which the streak was setup). There appears to be more growth than expected. Go ahead and test your latest culture, but also setup streak plates (and mock streaks) from your glycerol stocks. Let me know what you see. You can then go ahead and setup cultures. The spot test after 24 hrs using my newest M. smeg. P2FF (from stock #2) has definitely provided stronger clearings. This plate includes D29, phage buffer, and 2 other smeg phages from last year. After 24 hrs the streak with no bacteria shows no growth, and the new P2FF after 24 hrs has some growth. I did also start streak plates today using 3 different glycerol stocks. Edited 28 Aug, 2018 20:54 1.55Mb

Link to this post | posted 28 Aug, 2018 20:51

kaylafast

Viknesh Sivanathan
kaylafast
P2FF streak from stock #1
Incubation: 37 degrees C, 2 days (1 day photo unavailable)

I would be suspicious of this streak plate (and the culture from which the streak was setup). There appears to be more growth than expected. Go ahead and test your latest culture, but also setup streak plates (and mock streaks) from your glycerol stocks. Let me know what you see. You can then go ahead and setup cultures.

The spot test after 24 hrs using my newest M. smeg. P2FF (from stock #2) has definitely provided stronger clearings. This plate includes D29, phage buffer, and 2 other smeg phages from last year. After 24 hrs the streak with no bacteria shows no growth, and the new P2FF after 24 hrs has some growth.

I did also start streak plates today using 3 different glycerol stocks.

Edited 28 Aug, 2018 20:54

Link to this post \| posted 28 Aug, 2018 20:52
kaylafast	Control streak plate Incubation 37 degrees C, 24 hrs 1.64Mb

Link to this post \| posted 28 Aug, 2018 20:53
kaylafast	P2FF streak from stock #2 Incubation: 37 degrees C, 24 hrs 1.51Mb

Link to this post \| posted 28 Aug, 2018 20:56
debbie	Kayla, I think everything looks as I would expect. Do you think it is all correct now? debbie

Link to this post \| posted 28 Aug, 2018 21:05
kaylafast	Debbie Jacobs-Sera Kayla, I think everything looks as I would expect. Do you think it is all correct now? debbie So far I think we are back on track. I'll chunk the suspicious components. Thanks Debbie and Vic!

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D29 plaques barely visible on M. smeg