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Ruth Plymale posted in Tenure track Microbiology faculty position at Ouachita Baptist University in Arkansas
bburnes posted in Calling start in FC phage Phrampa
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bburnes posted in Calling start in FC phage Phrampa
Debbie Jacobs-Sera posted in Calling start in FC phage Phrampa
D29 plaques barely visible on M. smeg
Link to this post | posted 27 Aug, 2018 18:56 | |
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Kayla, Would you do dilutions of D29 and not just a single spot? You streak from P1FF streak from stock #2 looks like smeg. When you see 4 day colonies, are they dry and wrinkled? (as in, not smooth and glistening?) |
Link to this post | posted 28 Aug, 2018 20:51 | |
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Viknesh Sivanathankaylafast The spot test after 24 hrs using my newest M. smeg. P2FF (from stock #2) has definitely provided stronger clearings. This plate includes D29, phage buffer, and 2 other smeg phages from last year. After 24 hrs the streak with no bacteria shows no growth, and the new P2FF after 24 hrs has some growth. I did also start streak plates today using 3 different glycerol stocks. |
Link to this post | posted 28 Aug, 2018 20:52 | |
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Control streak plate Incubation 37 degrees C, 24 hrs |
Link to this post | posted 28 Aug, 2018 20:53 | |
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P2FF streak from stock #2 Incubation: 37 degrees C, 24 hrs |
Link to this post | posted 28 Aug, 2018 20:56 | |
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Kayla, I think everything looks as I would expect. Do you think it is all correct now? debbie |
Link to this post | posted 28 Aug, 2018 21:05 | |
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Debbie Jacobs-SeraSo far I think we are back on track. I'll chunk the suspicious components. Thanks Debbie and Vic! |