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ACMPhageHunters posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
cdshaffer posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
elongation factor P
| Link to this post | posted 01 Aug, 2018 20:14 | |
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Request for new function - elongation factor or translation elongation factor P to be more specific (Saguaro_77). Appprox 15 hits (> 90% probability and >92% coverage) in HHPRED to tranlation elongation factor P or elongation factor 5A which is the eukaryotic homolog. EF-P stimulates ribosomal peptidyltransferase activity/prevents ribosome stalling and is highly conserved. |
| Link to this post | posted 07 Aug, 2018 16:16 | |
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so in this particular case, the Saguaro gene is not long enough to be a full EF-P. It should be around 180aa, and this is only 107. EF-P was crystallized in sections, and this appears to be aligning to the OB domain, which is a beta-barrel. We might add elongation factor P to the functional list at some point, but I don't think this gene has enough evidence. http://www.pnas.org/content/101/26/9595.long |